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Timegated® Raman Technology

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About Timegated® Technology

 

For the past 30 years Raman spectroscopy has been widely used for numerous chemical research applications. Now, the new time-gated Raman technology broadens the application range of Raman to many new life science, geoscience and other applications where fluorescence or thermal interference has hampered successful Raman analyses. Fluorescence is an enemy? - Not anymore!

Don’t worry, time is on Your side!

Timegated® Raman Technology

Real fluorescence suppression

Time-gated Raman technology offers several advantages over conventional Raman spectrometers and opens up enormous potential for the use of this new technology in different application areas.

Key advantages:

  • Real fluorescence suppression
  • Time-resolved fluorescence information
  • Effective removal of ambient light and thermal emission interference

Download a White Paper about Rapid Milk Analysis with time-gated Raman spectroscopy.

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Whole milk Timegated® Raman spectra
Timegated® Raman signal pulse and fluorescence emission

Timegated® Raman technology

The Principle

Timegate Instruments’ patented breakthrough innovation is an affordable electrical gating solution using pulsed picosecond range lasers and new CMOS-SPAD (Single Photon Avalanche Diode) array detectors.

The picosecond range laser excitation source and a time-gated single photon counting array detector create a totally new type of spectrometer which is able to acquire Raman spectra with real fluorescence suppression. The system suppresses the fluorescence interference (which has a longer average delay) while capturing the instantaneous Raman scattering signal. It also enables the acquisition of time-resolved fluorescence spectra by sequentially sampling the emission pulses at different temporal positions. This approach simultaneously opens two windows for material characterization and provides valuable new information in several different application fields.

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Timegated® Raman technology

Example: Olive oil

Time-resolved emission spectrum of an olive oil measured with a Timegated® Raman instrument. The temporally short and spectrally multi-peaked Raman signal is clearly seen over the temporally and spectrally broad photoluminescence spectrum. In this figure, the origin of the time axis has no fundamental physical significance (the values are just the settings of the electronic delay generator).

Olive oil Timegated® Raman spectrum
Timegated® Raman signal pulse and fluorescence emission

Timegated® Raman technology

Leading the photonic revolution

The Achilles’ heel of conventional Raman technology is photoluminescence (including fluorescence and phosphorescence) interference. Photoluminescence is a competing phenomenon with Raman scattering and it can overlap or “swamp” Raman signals making the identification and quantification of materials impossible. With the new time-resolved Raman technology, we can now achieve real fluorescence suppression and redefine the ways Raman spectroscopy is exploited.

Read more about time-gating and other fluorescence suppression techniques on our blog.

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Timegated® Raman technology

Time-resolved total emission spectrum

Time-resolved total emission spectrum produced by a 532 nm pulsed excitation laser. This spectrum is the sum of the Raman and photoluminescence emission.

 

Total emission Timegated® Raman spectrum
Photoluminescence Timegated® Raman spectrum

Timegated® Raman technology

Time-resolved photoluminescence spectrum

Time-resolved background i.e. fluorescence surface contains fluorescence decay information and no Raman signals. Note that the temporally long “tail” of emission occurring well after the excitation. The 2D Raman spectrum is obtained by subtracting this fluorescence contribution from the overall signal. Fluorescence suppression is based on only collecting data from a time range containing a significant amount of Raman information while containing a minimum amount of the interfering background intensity.

Time-resolved Raman Spectrum

Time-resolved Raman spectrum

Time-resolved Raman spectrum without the background or baseline surface contains Raman signals without the photoluminescence interference. This spectrum is obtained when the baseline surface is subtracted from the total surface.

 

2D sum Timegated® Raman spectrum

Most of the photoluminescence interference (including fluorescence and phosphorescence) can be suppressed by collecting data from a time range containing a significant amount of Raman intensity while containing a minimal amount of photoluminescence intensity. The collected data can be summed up and presented as a 2D plot which contains a minimal amount of background interference.

 

Talcum process samples

Fluorescent samples have distinct spectral profiles. The difference can be observed quite easily when looking at the intensity vs time figures of samples with and without significant amounts of fluorescence emission.  The long tapering emission “tail” is caused by photoluminescence emission which has a long lifetime compared to Raman scattering.

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