Timegated® Technology
for biopharmaceutical industry
Have eyes inside your bioprocesses
Our innovation provides a solution to measure multiple critical process parameters, like nutrients and metabolites in real-time, and without manual sampling to see exactly what molecules you have in your bioprocesses and what is going on in the bioreactors.
Protein- and macromolecule-based drugs (i.e. biopharmaceuticals or biologics) are manufactured using biological-expression entities such as bacterial, mammalian or insect cells. Such conditions impose a significant biochemical and productional complexity for controlling and quantifying the processes and ensuring the efficacy and safety of the macromolecule products.
Our time-gated technology overcomes many of these difficulties while being a non-contact, robust, insensitive to water, suitable for automation as well as a flexible measurement technique. Timegated Raman resolves the current limitations in the production of biopharmaceuticals by providing time-resolved detection for fluorescence suppression, advanced pulsed laser and possibility to simultaneously determine the fluorescence lifetimes.
All of this is done to enable more effective biomanufacturing.
Benefits of Timegated® Raman in biopharmaceuticals
The online, real-time bioprocess monitoring offers several benefits to improve operational and manufacturing excellence. With our monitoring tools and services you can optimize your process constantly. This leads to
- Faster time to market through optimized process development and scale-up
- Improved product quality via QbD and controlled processing
- Reduced number of process failures via process monitoring and control
- Improved productivity through increased yields
- Reduced contamination risk and no volume pertubation (no need for manual sampling and off-line lab analyses)
- Reduced operational costs no analytical chemicals or consumables
- Reduced environmental impact with reduced carbon footprint
More about time-gated Raman technology in biopharmaceutical applications on our blog discussing Studies of Timegated® Raman in Life Sciences.
Successful pilots with Timegated® Raman Spectroscopy
Monitoring bioprocess in Testa Challenge
In the Testa Challenge we monitored the upstream and the downstream processes. We monitored the biprocess 24/7 in real-time. Even though the fluorescence interference increased simultaneously with the cell growth, we saw the charasteristic Raman signal.
The Raman spectra we collected during the challenge were great. We saw the gradual changes in the metabolites and the Raman spectra followed the bioprocess accurately. Read more about the Testa Challenge from the link below.
Monitoring ovalbumin production
Timegate Instruments in collaboration with VTT Bioprocess Engineering and Optical Measurements team carried out a pilot study where we monitored the bioprocess of ovalbumin production.
Timegate’s PicoRaman Spectrometer was installed in the bioreactor, and a continuous monitoring of the bioprocess was carried out. The results were amazing since time-gated Raman sensed the molecular level changes long before the more distinct morphological changes happened. Read more about the pilot from our news linked below.
