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Timegated® Raman Spectroscopy
for monitoring extracellular vesicles purification

Timegated® Raman Spectroscopy for EV Purification

 

Poster of time-gated Raman monitoring extracellular vesicles purification.

Extracellular vesicles (EVs) are nanosized particles released by virtually all cell types in their environment. EVs are involved in cell-to-cell communication and, thanks to this role as natural biocarriers, EVs hold a potential role as drug carriers, therapeutic agents, and diagnostic tools. Industrial-scale production of EVs requires technologies able to monitor the efficacy and efficiency of EV production, and purification.

In this poster, we propose a Timegated® Raman spectrometer as an in-line sensor to monitor a chromatography-based EV purification process. Our results suggest that the chemometric model based on Raman data can usefully monitor the purification process and discriminate between successful and failed runs.

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